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Resolvase

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Over 80% New & Buy It Now; This Is The New eBay. Find Vases Cobalt Blue Now The term resolvase is used to describe a related family of site-specific recombinases that function to excise (as a circle) a segment of DNA contained between two recombination sites (called res). The original resolvases were encoded by transposons of the Tn 3 -family Resolvase w, Transposonen. Redaktion Rolf Sauermost (Projektleiter) Doris Freudig (Redakteurin) Erweiterte Redaktio Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands

dict.cc | Übersetzungen für 'resolvase' im Englisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. Die sogenannte Tn3-Familie besitzt neben der Transposase auch eine Resolvase (tnpR) und enthält eine sogenannte res-Site. Transposasen der Tn3-Familie schneiden im Gegensatz zu denen der IS-Elemente nicht zwingend an den eigenen IR, sondern auch an entfernteren IR der gleichen Familie. Dadurch wird ein großes Stück Wirts-DNA eingeschlossen (Kointegrat), das durch die Resolvase in de Das Enzym Resolvase reguliert als Repressor die Transkription des Transposase-Gens und katalysiert die Auflösung der bei der Transposition entstehenden Cointegrat-Zwischenstruktur durch Rekombination der res-Loci. Durch IR sind die an den Enden von Tn 3 lokalisierten 38 bp invertierten Sequenzwiederholungen (inverted repeats) gekennzeichnet

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Tn3-Resolvase (Tn3R) ist ein [...] Protein, das den Austausch von DNA-Strängen bei der Rekombination innerhalb einer Bakterienzelle katalysiert. physik.uni-kiel.d Initially discovered as a repressor of transposase, resolvase also plays a role in facilitating Tn3 replication (Sherratt 1989). The transposon is flanked by a pair of 38bp inverted repeats RuvC is the resolvase, which cleaves the Holliday junction. RuvC proteins have been shown to form dimers in solution and its structure has been solved at 2.5A. It is thought to bind either on the open, DNA exposed face of a single RuvA tetramer, or to replace one of the two tetramers

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  1. Als Enzym des Bakteriophagen P1 hat Cre bei der Replikation seines Genoms eine Funktion als Resolvase. P1 integriert sein Genom nicht in das Wirtsgenom, sondern liegt als eigenständiges monomeres Molekül vor. Nach der Replikation ist es aber ein verbundenes Dimer und wird durch Cre voneinander getrennt 5 Funktion in der Biotechnologi
  2. Definition of resolvase in the Definitions.net dictionary. Meaning of resolvase. What does resolvase mean? Information and translations of resolvase in the most comprehensive dictionary definitions resource on the web
  3. ation of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresi
  4. resolvase ( plural resolvases ) ( biochemistry) Any of a group of enzymes that repress transposases
  5. Resolvase <p>This subsection of the <a href=http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection>Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry
  6. What are synonyms for resolvase? ENGLISH DICTIONARY; SYNONYMS; TRANSLATE; GRAMMAR . GRAMMAR A-Z ; SPELLING ; PUNCTUATION ; WRITING TIPS ; USAGE ; EXPLORE . WORD ORIGINS ; LANGUAGE QUESTIONS ; WORD LISTS; SPANISH DICTIONARY; More. GRAMMAR . GRAMMAR A-Z ; SPELLING ; PUNCTUATION ; WRITING TIPS ; USAGE ; EXPLORE . WORD ORIGINS ; LANGUAGE QUESTIONS ; WORD LISTS; SPANISH DICTIONARY ; Site language.

The present study aims to investigate the role of RHAU, an RBP with G4 resolvase activity in postnatal heart function through deletion of Rhau in the cardiomyocytes of postnatal mice. RHAU-deficient mice displayed progressive pathological remodeling leading to heart failure and mortality, and impaired neonatal heart regeneration. RHAU ablation reduced the protein levels but enhanced mRNA levels of Yap1 and Hexim1 that are important regulators for heart development and postnatal heart. Resolvase may fail to complete recombination, leaving species with double-strand breaks at one or both of the site Is, and a resolvase subunit attached via its active site serine residue (S10) to each DNA 5′-end (in the experiments below, the attached resolvase is degraded by treatment with protease). When the DNA is analysed without restriction enzyme digestion, products of.

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Resolvase, N terminal domain There are 61235 Resolvase domains in 61219 proteins in SMART's nrdb database. Click on the following links for more information Deutsch-Englisch-Übersetzungen für Resolvase im Online-Wörterbuch dict.cc (Englischwörterbuch) Hatfull GF, Grindley NDF (1986) Analysis of γδ resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res. Proc Natl Acad Sci USA 82:5429-5433 CrossRef Google Scholar. Hatfull GF, Grindley NDF (1988) Resolvases and DNA-invertases: a family of enzymes active in site-specific recombination. In: Kucherlapati R, Smith GR (eds) Genetic recombination.

Resolvase - an overview ScienceDirect Topic

résolvase translation in French - English Reverso dictionary, see also 'résolvent',resocialiser',résolu',rêvasser', examples, definition, conjugatio Genetic analysis of mutants in the γδ/Tn3 tnpR gene, which encodes the resolvase protein, suggested that resolvase regulates the frequency of transposition in addition to catalysing. RusA is another bacterial resolvase of phage origin. Like RuvC, it has been shown to bind the HJ in a tetrahedral conformation ( 35 , 36 ). However, based on the RusA structure in complex with two dsDNA fragments, a model was proposed that suggested that RusA exhibits yet another HJ binding mode in which the center of the HJ is open with unpairing of the bases to accommodate loop regions of. Gamma Delta Resolvase. Created by Vi Duong . The field of genetic engineering is constantly growing and changing at a tremendous pace. Recently, site-specific DNA recombination reactions have been adopted as a useful tool for genetic engineering 1.In contrast to homologous recombination seen in meiosis, site-specific DNA recombination reactions promote exchange of DNA strands at specific loci. aGenetik Klickerfragen: Die Resolvase RuvC kann in zwei möglichen Ebenen schneiden. Wie wird die Schnittebene fest gelegt? - e) Zufällig , Homologe Rekombination, aGenetik Klickerfragen kostenlos online.

Resolvase - Lexikon der Biologi

Then, the PCR products are melted and rehybridized, forming mismatched targets that can be cleaved by the Guide-it Resolvase (Figure 1, steps 2 and 3). Figure 1. Overview of the Guide-it Mutation Detection Kit method to confirm the presence of mutations in genomic DNA. Comparison of the Guide-it Mutation Detection Kit with a CEL nuclease-based assay . The key component of the Guide-it Mutation. Tn 3 transposition with Vinayak chali

Bacterial TransposonsRobert (&quot;Bob&quot;) Lloyd - The University of Nottingham

Resolvase acting on these sites cleaves the intact strand very rapidly, giving an abnormal half-site product which accumulates. We propose that these reactions mimic second-strand cleavage of an unmodified site. Cleavage occurs in a synapse of two sites, held together by a resolvase tetramer; cleavage at one site stimulates cleavage at the partner site. After cleavage of a nicked-site. Resolvase, N terminal domain Provide feedback. The N-terminal domain of the resolvase family (this family) contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase - see PF02796. Literature references . Yang W, Steitz TA; , Cell 1995;82:193-207.: Crystal structure of the site-specific. Request PDF | Resolvase | Resolvases are recombinases involved in site-specific recombination. The best studied are those from Tn. 3-family transposons that act as... | Find, read and cite all the. Fowlpox virus resolvase (Fpr) is an endonuclease that cleaves a broad range of branched DNA structures, including the Holliday junction (HJ), with little sequence-specificity. To better understand. Fowlpox virus resolvase (Fpr) is an endonuclease that cleaves a broad range of branched DNA structures, including the Holliday junction (HJ), with little sequence-specificity. To better understand the mechanisms underlying its relaxed substrate specificity, we determined the crystal structures of Fpr and that in a novel complex with HJ at 3.1-Å resolution. In the Fpr-HJ complex, two Fpr.

resolvase translation in English - French Reverso dictionary, see also 'resolve',resolved',resolute',resonate', examples, definition, conjugatio Recombinant Holliday Junction Resolvase RecU (RecU) Protein (His tag). Spezies: Staphylococcus aureus. Quelle: Hefe. Jetzt Produkt ABIN1510926 bestellen γδ resolvase catalyzes recombination within a complex nucleoprotein structure containing at least 12 resolvase subunits bound to two 114 bp res sites. The 2,3′ interaction between resolvase dimers is essential for synapsis and recombination. Using oriented resolvase heterodimers, we have identified half sites of res that must be occupied by a 2,3′-proficient protomer. For synapsis, only. CPV resolvase is dimer of RNase H superfamily domains related to Escherichia coli RuvC, with an active site lined by highly conserved acidic residues that bind metal ions. There are several intriguing structural differences between resolvase and RuvC, and a model of the CPV resolvase·Holliday junction complex provides insights into the consequences of these differences, including a plausible. dict.cc | Übersetzungen für 'resolvase' im Türkisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,.

Resolvase - Proteopedia, life in 3

  1. resolvase Übersetzung, Portugiesisch - Englisch Wörterbuch, Siehe auch 'resolver-se',relva',responsável',resolução', biespiele, konjugatio
  2. dict.cc | Übersetzungen für 'resolvase' im Kroatisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,.
  3. dict.cc | Übersetzungen für 'resolvase' im Polnisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,.
  4. Tn3 resolvase is a site-specific DNA recombinase, which catalyzes strand exchange in a synaptic complex containing twelve resolvase subunits and two res sites. Hyperactive mutants of resolvase can form a simpler complex (X synapse) containing a resolvase tetramer and two shorter DNA segments at which strand exchange takes place (site I). We have solved the low-resolution solution structure of.
  5. resolvase Übersetzung, Deutsch - Französisch Wörterbuch, Siehe auch 'resozialisieren',resolut',Resozialisierung',restlos', biespiele, konjugatio

Resolvase-katalysierte Sequenz-spezifische DNA-Rekombination in eukariotischen Zellen: Name des Anmelders: CARDION AG: Name des Einsprechenden: - Kammer: 3.3.04: Leitsatz: - Relevante Rechtsnormen: Patent Cooperation Treaty Art 17(3)(a) Patent Cooperation Treaty R 13(1) Patent Cooperation Treaty R 40(1) Schlagwörter: Uneinheitlichkeit (nein) Orientierungssatz: - Angeführte Entscheidungen: W dict.cc | Übersetzungen für 'resolvase' im Isländisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. In this study, we report on the transposition behavior of the mercury(II) resistance transposons Tn 502 and Tn 512 , which are members of the Tn 5053 family. These transposons exhibit targeted and oriented insertion in the par region of plasmid RP1, since par -encoded components, namely, the ParA resolvase and its cognate res region, are essential for such transposition dict.cc | Übersetzungen für 'resolvase' im Norwegisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,.

The RuvABC proteins of Escherichia coli play an important role in the processing of Holliday junctions during homologous recombination and recombinational repair. Mutations in the ruv genes have a moderate effect on recombination and repair in wild-type strains but confer pronounced recombination deficiency and extreme sensitivity to DNA-damaging agents in a recBC sbcBC background dict.cc | Übersetzungen für 'resolvase' im Esperanto-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. dict.cc | Übersetzungen für 'resolvase' im Finnisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. translation and definition resolvase, Dictionary English-English online. resolvase Definitions. en.wiktionary.org (biochemistry) Any of a group of enzymes that repress transposases. en.wiktionary.2016 [noun] Any of a group of enzymes that repress transposases Show declension of resolvase ( )) Example sentences with resolvase, translation memory. patents-wipo. A method includes silencing.

Holliday Junction Resolvase MOC1 Maintains Plastid and Mitochondrial Genome Integrity in Algae and Bryophytes. Yusuke Kobayashi, Yusuke Kobayashi a College of Science, Graduate School of Science and Engineering, Ibaraki University, Bunkyo, Mito, Ibaraki 310-8512, Japan. b Department of Gene Function and Phenomics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. https. dict.cc | Übersetzungen für 'resolvase' im Deutsch-Tschechisch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. The structures of two mutants of the site-specific recombinase, γδ resolvase, that form activated tetramers have been determined. One, at 3.5-Å resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-Å resolution. Comparisons of the four known tetrameric resolvase structures show.

dict.cc | Übersetzungen für 'resolvase' im Portugiesisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. dict.cc | Übersetzungen für 'resolvase' im Ungarisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. The plasmide to be stabilised contains a recognition site for a site-specific recombinase which is generally a resolvase.. dict.cc | Übersetzungen für 'resolvase' im Schwedisch-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. dict.cc | Übersetzungen für 'resolvase' im Deutsch-Dänisch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,.

Übersetzung für 'redevance' im kostenlosen Französisch-Deutsch Wörterbuch und viele weitere Deutsch-Übersetzungen We selected the telomere resolvase, TelA from Agrobacterium tumefaciens as it is the closest to ResT in both size and sequence homology and has available partial structural data. We have demonstrated that TelA also has the ability to promote the annealing of complementary ssDNA, and removal of the N-terminal domain of TelA produces a mutant deficient for annealing activity while still. dict.cc | Übersetzungen für 'resolvase' im Deutsch-Bulgarisch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. Resolvase-katalysierte, Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen . German Patent DE19956513 . Kind Code: A1 . Abstract: The invention relates to a method for the sequence-specific recombination of DNA in eukaryotic cells, comprising the following steps: introducing a first DNA sequence which contains a res sequence into a cell, introducing a copy of the first DNA sequence.

resolvase Übersetzung Englisch-Deutsc

  1. Thank you for submitting your work entitled Human Holliday junction resolvase GEN1 uses a chromodomain for efficient DNA recognition and cleavage for consideration by eLife. Your article has been reviewed by three peer reviewers, one of whom is a member of our Board of Reviewing Editors (Volker Dötsch). The following reviewer has agreed to reveal their identity: Stephen West. The evaluation.
  2. Lee, Shun-Hsiao (2017): Structural and biochemical characterization of human GEN1 holliday junction resolvase. Dissertation, LMU München: Fakultät für Chemie und Pharmazie Vorschau. PDF Lee_Shun-Hsiao.pdf 11MB: DOI: 10.5282/edoc.23326. URN: urn:nbn:de:bvb:19-233267. Dokumententyp: Dissertationen (Dissertation, LMU München) Themengebiete: 500 Naturwissenschaften und Mathematik 500.
  3. Ausspracheführer: Lernen Sie Resolvase auf Englisch muttersprachlich auszusprechen. Englische Übersetzung von Resolvase

Transposon - Wikipedi

The structure of a synaptic intermediate of the site-specific recombinase γδ resolvase covalently linked through Ser10 to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA dict.cc | Übersetzungen für 'resolvase' im Latein-Deutsch-Wörterbuch, mit echten Sprachaufnahmen, Illustrationen, Beugungsformen,. Resolvase. definitions. (biochemistry) Any of a group of enzymes that repress transposases We recently identified Monokaryotic Chloroplast1 (MOC1) as a plastid DNA HJ resolvase in algae and plants. Although Cruciform cutting endonuclease1 (CCE1) was identified as a mitochondrial DNA HJ resolvase in yeasts, homologs or other mitochondrial HJ resolvases have not been identified in other eukaryotes. Here, we demonstrate that MOC1 depletion in the green alg

The RuvABC Resolvase Is Indispensable for Recombinational Repair in sbcB15 Mutants of Escherichia coli Davor Zahradka , Ksenija Zahradka , Mirjana Petranović , Damir Đermić , Krunoslav Brčić-Kosti In the res/resolvase system and in other microbial re­ combination systems (but not in the immunoglobulin system), religation of left ends to right ends is require The structures of two mutants of the site-specific recombinase, γδ resolvase, that form activated tetramers have been determined. One, at 3.5-Å resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-Å resolution. Comparisons of the four known tetrameric resolvase structures show that the subunits interact through the formation of a common core of four helices. The N-terminal. Amplify genomic regions directly from cells without the need for DNA purification, and detect mutations with the highly efficient Guide-it Resolvase enzyme . Faster and more efficient than CEL nuclease-based assays: The Guide-it mutation detection protocol is several hours shorter, more sensitive, and less prone to non-specific cleavag Resolvase and nicking activities of AtGEN1 and AtSEND1 on plasmid pIR9. A, The plasmid pIR9 features an inverted repeat, which forms a HJ stabilized by negative supercoiling. A single incision leads to relaxation and reabsorption of the HJ if the junction is not stabilized by the DNA-protein complex. The resulting nicked circular (n.c.) plasmid, therefore, is a nicking product. Resolution into.

RecA ist ein 38 Kilodalton großes Protein in Escherichia coli, welches in der Reparatur und Erhaltung von DNA eine Rolle spielt. Man hat in allen Spezies, in welchen danach gesucht wurde, ein Homolog zum E. coli RecA gefunden. Das homologe Protein des Menschen ist Rad51; im Allgemeinen findet sich bei Eukaryoten (komplex-zellulären Organismen) neben Rad51 auch DMC1 als homologes Protein, bei. Tn3 resolvase or is one out of three proteins that are encoded on the 4957 bp Tn3 transposon.Apart from Tn3 resolvase or tnpR, this transposon also carries a transposase or tnpA and a β-lactamase Bla that confers resistance to β-lactam antibiotics.Initially discovered as a repressor of transposase, resolvase also plays a role in facilitating Tn3 replication (Sherratt 1989) To determine the physiologically important features of site-specific recombination by Escherichia coli Tn3 resolvase we analyzed the salient properties of the reaction in vivo. A two-plasmid system in which one plasmid served as substrate while the other encoded both resolvase and a thermolabile repressor of resolvase transcription provided controlled, synchronous recombination. Recombination. We recently identified Monokaryotic Chloroplast1 (MOC1) as a plastid DNA HJ resolvase in algae and plants. Although Cruciform cutting endonuclease1 (CCE1) was identified as a mitochondrial DNA HJ resolvase in yeasts, homologs or other mitochondrial HJ resolvases have not been identified in other eukaryotes. Here, we demonstrate that MOC1 depletion in the green alga Chlamydomonas reinhardtii and the moss Physcomitrella patens induced ectopic recombination between short dispersed repeats in.

A small, diverse class of enzyme, referred to as telomere resolvases are responsible for this activity. To date, three telomere resolvases have been well-characterized including TelK from Klebsiella phage KO2, TelA from the Agrobacterium genus, and ResT from Borrelia species. Of these ResT is the best biochemically characterized telomere resolvase. Previous studies have revealed that aside from the expected property of telomere resolution, ResT can anneal ssDNA and also possesses an ATP. Resolvase bound to individual res sites protects approximately −0.5 supercoil per site from relaxation by a topoisomerase, whereas the formation of the synaptosome protects −3 supercoils and condenses the associated DNA to a supercoil density 2.5 times that of the non‐complexed substrate. Although recombination requires two directly repeated res sites, both direct and inverted sites form.

Here, we present the crystal structure of human HJ resolvase GEN1 complexed with DNA at 3.0 Å resolution. The GEN1 core is similar to other Rad2/XPG nucleases. However, unlike other members of the superfamily, GEN1 contains a chromodomain as an additional DNA interaction site. Chromodomains are known for their chromatin-targeting function in chromatin remodelers and histone(de)acetylases but they have not previously been found in nucleases. The GEN1 chromodomain directly contacts DNA and. ![Figure][1] CREDIT: LI ET AL. The site-specific serine recombinase, resolvase, catalyzes recombination between two sites on negatively supercoiled DNA. This process requires double-strand cleavage at each site, strand exchange between the two sites, and religation. Li et al. (p. [1210][2], published online 30 June 2005) provide insight into how this occurs by reporting a 3.4 angstrom. Lee, Shun-Hsiao (2017): Structural and biochemical characterization of human GEN1 holliday junction resolvase. Dissertation, LMU München: Fakultät für Chemie und Pharmazie Dissertation, LMU München: Fakultät für Chemie und Pharmazi The amplicon is then melted and hybridized to form the mismatched targets for cleavage by the Guide-it Resolvase. Sufficient material is provided for 100 amplification and cleavage reactions. Notice to purchaser . Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or.

Transposonen - Lexikon der Biologi

View application images and datasheets for 44 anti Holliday-junction-resolvase-RuvC Antibody antibodies from 6 leading antibody suppliers, plus reviews and the top related antibodie resolvase. Interpretation Translation  resolvase. A gene encoded by a transposon that can catalyze a second stage of transposition as well as participate in the regulation of its own. Resolvase binds to each res site, and then the bound sites are brought together to form a structure ~10 nm in diameter. Changes in supercoiling occur during the reaction, and DNA is bent at the res sites by the binding of transposase. Resolution is a nonreplicative reaction; bonds are broken and rejoined without demand for input of energy. The products identify an intermediate stage in.

Resolution of single and double Holliday junctionRegulated Crossing-Over Requires Inactivation of Yen1/GEN1

resolvase - Deutsch-Übersetzung - Linguee Wörterbuc

Tn3 transposon - Wikipedi

RuvABC - Wikipedi

  1. Icelandic Translation for resolvase - dict.cc English-Icelandic Dictionar
  2. For more information please use the links below or search the forum for resolvase! Report missing translation EN > SQ (resolvase is English, Albanian term is missing) SQ > EN (resolvase is Albanian, English term is missing)... or add translation directly. Albanian Links: English Links: Google; Wikipedia; Wiktionary; Ectaco; GoogleTrans ; Fjalorshqip; Fjalorthi; Customize Links. Goog
  3. Cre-Rekombinase - DocCheck Flexiko
  4. What does resolvase mean? - Definitions
ResT, a Telomere Resolvase Encoded by the Lyme DiseaseTetrameric Structure of a Serine Integrase CatalyticArchitecture of a Serine Recombinase-DNA RegulatoryASMscience | The Movement of Tn3-LikeFrontiers | Novel Mobilizable Genomic Island GEI-D18ADprB Facilitates Inter- and Intragenomic Recombination in
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